Markus Klotz GmbH
diagnostics Biochips are used for various purposes to obtain cellular
and genetic information, as testing system for protein analysis, for
immunological diseases or for testing foodstuff. In all cases we
are dealing with two-dimensional matrix whose elements are charged with
varying nucleotide sequences (ogligonucleotides) or c-DNA fragments.
After hybridization with fluorescence tagged RNA from the sample
material only those matrix elements having a certain agreement with the
genetic code will show a fluorescence.
Through the application
of the modified particle counter “Syringe®” the rigid structure with
complicated manufacturing of chips is broken by using small globules of
various sizes between 2µm und 150µm which can be accurately measured
in-flow by means of laser light. The globules are coated accordingly
and depending on the test different size class are hybridized together
with the sample material. Allocation is given, since the size and
corresponding fluorescence is recorded during each measuring cycle. This
method provides a simpler preparation of the globules according to
quality criteria, less efforts for testing and a more flexible and
customized coordination of the problems to be examined.